Aboveground Total and Green Biomass of Dryland Shrub Derived from Terrestrial Laser Scanning

Sagebrush (Artemisia tridentata), a dominant shrub species in the sagebrush-steppe ecosystem of the western US, is declining from its historical distribution due to feedbacks between climate and land use change, fire, and invasive species. Quantifying aboveground biomass of sagebrush is important for assessing carbon storage and monitoring the presence and distribution of this rapidly changing dryland ecosystem. Models of shrub canopy volume, derived from terrestrial laser scanning (TLS) point clouds, were used to accurately estimate aboveground sagebrush biomass. Ninety-one sagebrush plants were scanned and sampled across three study sites in the Great Basin, USA. Half of the plants were scanned and destructively sampled in the spring (n = 46), while the other half were scanned again in the fall before destructive sampling (n = 45). The latter set of sagebrush plants was scanned during both spring and fall to further test the ability of the TLS to quantify seasonal changes in green biomass. Sagebrush biomass was estimated using both a voxel and a 3-D convex hull approach applied to TLS point cloud data. The 3-D convex hull model estimated total and green biomass more accurately (R2 = 0.92 and R2 = 0.83, respectively) than the voxel-based method (R2 = 0.86 and R2 = 0.73, respectively). Seasonal differences in TLS-predicted green biomass were detected at two of the sites (p \u3c 0.001 and p = 0.029), elucidating the amount of ephemeral leaf loss in the face of summer drought. The methods presented herein are directly transferable to other dryland shrubs, and implementation of the convex hull model with similar sagebrush species is straightforward

Assessing a Multi-Platform Data Fusion Technique in Capturing Spatiotemporal Dynamics of Heterogeneous Dryland Ecosystems in Topographically Complex Terrain

Water-limited ecosystems encompass approximately 40% of terrestrial land mass and play a critical role in modulating Earth’s climate and provisioning ecosystem services to humanity. Spaceborne remote sensing is a critical tool for characterizing ecohydrologic patterns and advancing the understanding of the interactions between atmospheric forcings and ecohydrologic responses. Fine to medium scale spatial and temporal resolutions are needed to capture the spatial heterogeneity and the temporally intermittent response of these ecosystems to environmental forcings. Techniques combining complementary remote sensing datasets have been developed, but the heterogeneous nature of these regions present significant challenges. Here we investigate the capacity of one such approach, the Spatial and Temporal Adaptive Reflectance Fusion Model (STARFM) algorithm, to map Normalized Difference Vegetation Index (NDVI) at 30 m spatial resolution and at a daily temporal resolution in an experimental watershed in southwest Idaho, USA. The Dry Creek Experimental Watershed captures an ecotone from a sagebrush steppe ecosystem to evergreen needle-leaf forests along an approximately 1000 m elevation gradient. We used STARFM to fuse NDVI retrievals from the MODerate-resolution Imaging Spectroradiometer (MODIS) and Landsat during the course of a growing season (April to September). Specifically we input to STARFM a pair of Landsat NDVI retrievals bracketing a sequence of daily MODIS NDVI retrievals to yield daily estimates of NDVI at resolutions of 30 m. In a suite of data denial experiments we compared these STARFM predictions against corresponding Landsat NDVI retrievals and characterized errors in predicted NDVI. We investigated how errors vary as a function of vegetation functional type and topographic aspect. We find that errors in predicting NDVI were highest during green-up and senescence and lowest during the middle of the growing season. Absolute errors were generally greatest in tree-covered portions of the watershed and lowest in locations characterized by grasses/bare ground. On average, relative errors in predicted average NDVI were greatest in grass/bare ground regions, on south-facing aspects, and at the height of the growing season. We present several ramifications revealed in this study for the use of multi-sensor remote sensing data for the study of spatiotemporal ecohydrologic patterns in dryland ecosystems

Fearscapes: Mapping Functional Properties of Cover for Prey with Terrestrial LiDAR

Heterogeneous vegetation structure can create a variable landscape of predation risk—a fearscape—that influences the use and selection of habitat by animals. Mapping the functional properties of vegetation that influence predation risk (e.g., concealment and visibility) across landscapes can be challenging. Traditional ground-based measures of predation risk are location specific and limited in spatial resolution. We demonstrate the benefits of terrestrial laser scanning (TLS) to map the properties of vegetation structure that shape fearscapes. We used TLS data to estimate the concealment of prey from multiple vantage points, representing predator sightlines, as well as the visibility of potential predators from the locations of prey. TLS provides a comprehensive data set that allows an exploration of how habitat changes may affect prey and predators. Together with other remotely sensed imagery, TLS could facilitate the scaling up of fearscape analyses to promote the management and restoration of landscapes

Scaling Up Sagebrush Chemistry with Near-Infrared Spectroscopy and UAS-Acquired Hyperspectral Imagery

Sagebrush ecosystems (Artemisia spp.) face many threats including large wildfires and conversion to invasive annuals, and thus are the focus of intense restoration efforts across the western United States. Specific attention has been given to restoration of sagebrush systems for threatened herbivores, such as Greater Sage-Grouse (Centrocercus urophasianus) and pygmy rabbits (Brachylagus idahoensis), reliant on sagebrush as forage. Despite this, plant chemistry (e.g., crude protein, monoterpenes and phenolics) is rarely considered during reseeding efforts or when deciding which areas to conserve. Near-infrared spectroscopy (NIRS) has proven effective in predicting plant chemistry under laboratory conditions in a variety of ecosystems, including the sagebrush steppe. Our objectives were to demonstrate the scalability of these models from the laboratory to the field, and in the air with a hyperspectral sensor on an unoccupied aerial system (UAS). Sagebrush leaf samples were collected at a study site in eastern Idaho, USA. Plants were scanned with an ASD FieldSpec 4 spectroradiometer in the field and laboratory, and a subset of the same plants were imaged with a SteadiDrone Hexacopter UAS equipped with a Rikola hyperspectral sensor (HSI). All three sensors generated spectral patterns that were distinct among species and morphotypes of sagebrush at specific wavelengths. Lab-based NIRS was accurate for predicting crude protein and total monoterpenes (R2 = 0.7–0.8), but the same NIRS sensor in the field was unable to predict either crude protein or total monoterpenes (R2 \u3c 0.1). The hyperspectral sensor on the UAS was unable to predict most chemicals (R2 \u3c 0.2), likely due to a combination of too few bands in the Rikola HSI camera (16 bands), the range of wavelengths (500–900 nm), and small sample size of overlapping plants (n = 28–60). These results show both the potential for scaling NIRS from the lab to the field and the challenges in predicting complex plant chemistry with hyperspectral UAS. We conclude with recommendations for next steps in applying UAS to sagebrush ecosystems with a variety of new sensors

Data for Aboveground Biomass Estimates of Sagebrush Using Terrestrial and Airborne LiDAR Data in a Dryland Ecosystem

Vegetation biomass estimates across drylands at regional scales are critical for ecological modeling, yet the low-lying and sparse plant communities characterizing these ecosystems are challenging to accurately quantify and measure their variability using spectral-based aerial and satellite remote sensing. To overcome these challenges, multi-scale data including field-measured biomass, terrestrial laser scanning (TLS) and airborne laser scanning (ALS) data, were combined in a hierarchical modeling framework. Data derived at each scale were used to validate an increasingly broader index of sagebrush (Artemisia tridentata) aboveground biomass. First, two automatic crown delineation methods were used to delineate individual shrubs across the TLS plots. Second, three models to derive shrub volumes were utilized with TLS data and regressed against destructively-sampled individual shrub biomass measurements. Third, TLS-derived biomass estimates at 5 m were used to calibrate a biomass prediction model with a linear regression of ALS-derived percent vegetation cover (adjusted R2 = 0.87, p \u3c 0.001, RMSE = 3.59 kg). The ALS prediction model was applied to the study watershed and evaluated with independent TLS plots (adjusted R2 = 0.55, RMSE = 4.01 kg, normalized RMSE = 35%). The biomass estimates at the scale of 5 m is sufficient for capturing the variability of biomass needed to initialize models to estimate ecosystem fluxes, and the contiguous estimates across the watershed support analyzing patterns and connectivity of these dynamics. Our model is currently optimized for the sagebrush-steppe environment at the watershed scale and may be readily applied to other shrub-dominated drylands, and especially the Great Basin, U.S., which extends across five western states. Improved derived metrics from ALS data and collection of additional TLS data to refine the relationship between TLS-derived biomass estimates and ALS-derived models of vegetation structure, will strengthen the predictive power of our model and extend its range to similar shrubland ecosystems. The corresponding data set is composed of an attributes list in comma separate value format (.csv)

Sharp Transition of End-Grafted PNIPAM Studied by Interfacial Force Microscopy

Poly N-isopropylacrylamide (PNIPAM) experiences a transition from a swollen hydrophilic state to a collapsed hydrophobic state at the lower critical solution temperature (LCST) in solution. Based on the transition behavior, the PNIPAM has been end-grafted to a surface for future applications of the polymer, such as in microfluidics, drug delivery, and tissue engineering. Recent studies have shown that the end-grafted PNIPAM does not collapse above the LCST at low grafting density and at low molecular weight. On the other hand, a sharp transition is observed with intermediate grafting density and intermediate molecular weight. To explore the transition of PNIPAM in the crossover region between low and intermediate molecular configuration, we investigated the PNIPAM end-grafted to an oxidized silicon surface at low molecular weight and intermediate grafting density. This was achieved using interfacial force microscopy, and was observed as solution temperature varies. We measured the force as a function of distance between a PNIPAM polymer brush and a tip coated with a hydrophobic molecule of octadecyltrichlorosilane. Repulsive forces were observed to exponentially decrease with the distance, corresponding to a swollen, hydrated state at temperatures below the LCST. Above the LCST, adhesive forces were present in the force-distance curves, thus consistent with a collapsed, hydrophobic state. The results suggest that, in the crossover region, the end-grafted PNIPAM experiences a sharp transition as the temperature increases

Aboveground Biomass Estimates of Sagebrush Using Terrestrial and Airborne LiDAR Data in a Dryland Ecosystem

Vegetation biomass estimates across drylands at regional scales are critical for ecological modeling, yet the low-lying and sparse plant communities characterizing these ecosystems are challenging to accurately quantify and measure their variability using spectral-based aerial and satellite remote sensing. To overcome these challenges, multi-scale data including field-measured biomass, terrestrial laser scanning (TLS) and airborne laser scanning (ALS) data, were combined in a hierarchical modeling framework. Data derived at each scale were used to validate an increasingly broader index of sagebrush (Artemisia tridentata) aboveground biomass. First, two automatic crown delineation methods were used to delineate individual shrubs across the TLS plots. Second, three models to derive shrub volumes were utilized with TLS data and regressed against destructively-sampled individual shrub biomass measurements. Third, TLS-derived biomass estimates at 5 m were used to calibrate a biomass prediction model with a linear regression of ALS-derived percent vegetation cover (adjusted R2 = 0.87, p \u3c 0.001, RMSE = 3.59 kg). The ALS prediction model was applied to the study watershed and evaluated with independent TLS plots (adjusted R2 = 0.55, RMSE = 4.01 kg, normalized RMSE = 35%). The biomass estimates at the scale of 5 m is sufficient for capturing the variability of biomass needed to initialize models to estimate ecosystem fluxes, and the contiguous estimates across the watershed support analyzing patterns and connectivity of these dynamics. Our model is currently optimized for the sagebrush-steppe environment at the watershed scale and may be readily applied to other shrub-dominated drylands, and especially the Great Basin, U.S., which extends across five western states. Improved derived metrics from ALS data and collection of additional TLS data to refine the relationship between TLS-derived biomass estimates and ALS-derived models of vegetation structure, will strengthen the predictive power of our model and extend its range to similar shrubland ecosystems

Estimation of Big Sagebrush Leaf Area Index with Terrestrial Laser Scanning

Accurate monitoring and quantification of the structure and function of semiarid ecosystems is necessary to improve carbon and water flux models that help describe how these systems will respond in the future. The leaf area index (LAI, m2m-2) is an important indicator of energy, water, and carbon exchange between vegetation and the atmosphere. Remote sensing techniques are frequently used to estimate LAI, and can provide users with scalable measurements of vegetation structure and function. We tested terrestrial laser scanning (TLS) techniques to estimate LAI using structural variables such as height, canopy cover, and volume for 42 Wyoming big sagebrush (Atremisia tridentate subsp. wyomingensis Beetle & Young) shrubs across three study sites in the Snake River Plain, Idaho, USA. The TLS-derived variables were regressed against sagebrush LAI estimates calculated using specific leaf area measurements, and compared with point-intercept sampling, a field method of estimating LAI. Canopy cover estimated with the TLS data proved to be a good predictor of LAI (r2=0.73). Similarly, a convex hull approach to estimate volume of the shrubs from the TLS data also strongly predicted LAI (r2=0.76), and compared favorably to point-intercept sampling (r2=0.78), a field-based method used in rangelands. These results, coupled with the relative ease-of-use of TLS, suggest that TLS is a promising tool for measuring LAI at the shrub-level. Further work should examine the structural measures in other similar shrublands that are relevant for upscaling LAI to the plot-level (i.e., hectare) using data from TLS and/or airborne laser scanning and to regional levels using satellite-based remote sensing

Evaluation of micro-GPS receivers for tracking small-bodied mammals.

GPS telemetry markedly enhances the temporal and spatial resolution of animal location data, and recent advances in micro-GPS receivers permit their deployment on small mammals. One such technological advance, snapshot technology, allows for improved battery life by reducing the time to first fix via postponing recovery of satellite ephemeris (satellite location) data and processing of locations. However, no previous work has employed snapshot technology for small, terrestrial mammals. We evaluated performance of two types of micro-GPS (< 20 g) receivers (traditional and snapshot) on a small, semi-fossorial lagomorph, the pygmy rabbit (Brachylagus idahoensis), to understand how GPS errors might influence fine-scale assessments of space use and habitat selection. During stationary tests, microtopography (i.e., burrows) and satellite geometry had the largest influence on GPS fix success rate (FSR) and location error (LE). There was no difference between FSR while animals wore the GPS collars above ground (determined via light sensors) and FSR generated during stationary, above-ground trials, suggesting that animal behavior other than burrowing did not markedly influence micro-GPS errors. In our study, traditional micro-GPS receivers demonstrated similar FSR and LE to snapshot receivers, however, snapshot receivers operated inconsistently due to battery and software failures. In contrast, the initial traditional receivers deployed on animals experienced some breakages, but a modified collar design consistently functioned as expected. If such problems were resolved, snapshot technology could reduce the tradeoff between fix interval and battery life that occurs with traditional micro-GPS receivers. Our results suggest that micro-GPS receivers are capable of addressing questions about space use and resource selection by small mammals, but that additional techniques might be needed to identify use of habitat structures (e.g., burrows, tree cavities, rock crevices) that could affect micro-GPS performance and bias study results